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Morning administration in older populations produces minimal GH elevation due to compounding factors: lower pituitary GH stores, higher basal somatostatin tone, and reduced receptor density

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Afterwards, cells were blocked, permeabilized and incubated with a primary mouse monoclonal antibody to H2AX (Ser-139) (Upstate Biotechnology, Lake Placid, NY, USA) diluted 1:500 for 16 h at 4 C, followed by incubation with anti-mouse FITC secondary antibody (Sigma-Aldrich) diluted 1:200 for 2 h at room temperature, or with a mouse anti-active caspase 3 (BD, Pharmigen, San Diego, CA, USA) diluted 1:50 for 16 h at 4 C

Cell viability and Melanin quantification assay Normal human epidermal melanocytes (Cascade Biologics TM (Portland, OR)) were cultured in HMGS (Cascade Biologics) supplemented Medium 254